You can dispose of your appreciated cryptocurrency, thereby triggering a gain that you can invest in your Qualified Opportunity Fund (QOF) to obtain Opportunity Zone benefits (assuming that you hold your cryptocurrency as a capital asset). If instead you simply used appreciated cryptocurrency to acquire an interest in your QOF, the portion of the QOF interest acquired for such appreciation will not be treated as a qualifying investment. In addition, the remaining portion of the QOF interest will not be a qualifying investment either, except to the extent that you have gains from other sources that you can defer.